Coding

Part:BBa_K4229064

Designed by: Nikita Edel   Group: iGEM22_Freiburg   (2022-09-30)


mTurquoise2 with C-Terminal SnoopCatcher

mTurquoise (together with mVenus2,[BBa_K4229064]) was used as a reporter by fusing it with a SpyCatcher, enabling recruitment by the SpyTag. We used this fusion protein to test the functionality of our compartments: the minimal (BBa_K4229047) and full wiffleball (BBa_K4229049), and SPD5 (BBa_K4229078). All compartmentalisation proteins have an N-terminal SpyTag. For experimental data please refer to the data listed in Biobrick BBa_K4229067.



Principle of catching proteins via Spy/Snp-Catcher by T1 and their incorporation into the BMCs, illustrated as an example of incorporating mVenus in the full wiffleball



















Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 763
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 763
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 763
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 763
    Illegal AgeI site found at 28
    Illegal AgeI site found at 924
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None